Everything for the practical, in one place · Dr Daniel Mompel Riera
This is the practical. It tells you what you are measuring, how to make the standards, the method step by step, and exactly what you are assessed on. Nothing else on this page will make sense until you have read it.
Download the worksheet (Word) ↓And one more thing before you arrive: install Vernier Spectral Analysis on the laptop you are bringing. It is free, and there are versions for Windows, Mac, iPad and Android. On a Chromebook, open spectralanalysis.app in Chrome instead. If thirty people download it at once when they sit down, that is ten minutes of the practical gone.
Once you have your readings, these turn them into a graph and an answer.
Type your absorbance readings into the yellow cells on the Your data tab. The graph, the line of best fit, the gradient and R² all appear on their own. There is also a worked example to check yourself against, and a tab explaining how to build the chart in Excel.
Does the same analysis as the workbook, in one click. Save it in the same folder as your workbook, open it in RStudio and press Source. You do not need this to complete the practical.
Entirely optional, and not needed for the practical — read it once the worksheet makes sense.
An introduction to R, the free tool that biology, medicine, economics and psychology all use to handle data. It walks through the same calibration curve, one step at a time, with real R running in your browser — or download the script and run it in RStudio. Nothing in it assumes you have written a line of code before.